Purified polyclonal anti‐phenylurea antibodies for an improved immunoaffinity chromatography
نویسندگان
چکیده
منابع مشابه
Presence of matrix-specific antibodies in affinity-purified polyclonal antibodies.
In general, antigen affinity columns made with commercially prepared activated affinity supports bind antibody specific for the coupled antigen. Nonetheless, in some cases affinity purification may yield antibodies to molecules other than the molecule of interest. In this report, we demonstrate such an occurrence: an antibody which adsorbs to an Affi-Prep 10 affinity matrix was found in the ser...
متن کاملProduction and characterization of polyclonal antibodies to sulfamethazine and their potential use in immunoaffinity chromatography for urine sample pre-treatment.
An immunoaffinity chromatographic (IAC) method for isolating sulfamethazine (SMZ) from incurred urine samples was developed. This was achieved by (i) generating polyclonal antibodies that recognize equally well SMZ and its major urinary metabolites, (ii) evaluating in an ELISA procedure the influence of methanol, salt and pH on the antigen-antibody interaction in order to determine the optimum ...
متن کاملPurification of a polyclonal antibody against CD147 for ELISA using antigen-immunoaffinity chromatography
The immunoglobulin superfamily member CD147 is a widely expressed glycoprotein that occurs in both a membrane‑spanning and soluble form. Sandwich ELISA is a powerful tool for analyzing soluble antigens. The aim of the present study was to obtain a highly specific polyclonal antibody against human CD147 that can be used for sandwich ELISA analysis. Expression of recombinant CD147 by a eukaryotic...
متن کاملImmunoaffinity Chromatography: A Review
Affinity chromatography is a high resolution, high capacity, and one of the most powerful and diverse methods for separating proteins and other biological molecules of interest on the basis of a highly specific, reversible biological interaction between two molecules: an affinity ligand attached to a solid matrix to create a stationary phase, and a target molecule in a mobile phase. Specificall...
متن کاملPolyclonal Antibodies for Detection of Witchesâ Broom Disease of Lime
The witches’ broom disease of lime trees is a destructing disease caused by the bacterium Candidatus <span style="font-variant: normal; fon...
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ژورنال
عنوان ژورنال: Food and Agricultural Immunology
سال: 1998
ISSN: 0954-0105,1465-3443
DOI: 10.1080/09540109809354984